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Home » Blog » The Right Way to Do a Urine Collection and Stop Making Stones

The Right Way to Do a Urine Collection and Stop Making Stones

Jill Harris, LPN, CHC Avatar

Written by

Jill Harris, LPN, CHC

Updated

April 17, 2026

If you’ve had a kidney stone, there’s a good chance someone told you to drink more water and left it at that.

But if you really want to know why you are making stones, a urine collection matters.

This is one of the most useful tests we have. It can tell us whether your urine volume is too low, whether your calcium is too high, whether oxalate is high, whether citrate is low, and more.

That means we can stop guessing and start making the right diet changes for your body.

Too many people make a stone, get scared, and start cutting out foods left and right without ever knowing what the real problem is. They stop eating regular meals, throw out perfectly fine foods, start adding things they don’t need (like lemons) to everything, and follow advice from anybody with a pulse and a Facebook account.

That is how people end up overwhelmed, confused, and eating five foods while still making stones.

A urine collection gives us real answers. Different urine problems need different solutions. What helps one person may not help you, and that is exactly why this test matters so much.

The good news is that the test is not hard. But you do need to do it correctly.

For a 24-hour urine collection, choose a day that is normal for you. Not a vacation day. Not a holiday. Not the day you suddenly decide to become the healthiest person in America.

You want a regular day that reflects how you usually eat and drink, because the whole point is to see what your body is doing in real life.


Starting Your Collection

Your collection does not start with a middle-of-the-night bathroom trip. It starts when you wake up and begin your day.

That first morning urine gets flushed, not saved. But the time you urinate is your official start time, so write it down.

Why? Because that urine was made overnight, before the collection officially began. It reflects what was happening in your body before the test started — including food and drink from the day before. We want to begin with an empty bladder so we can measure everything your body produces over the next full 24 hours.

Then, the next morning at that same time, you do collect that urine — because it was made during the collection period. That final sample completes the test.

To make it simple: flush the first morning urine, write down the time, collect everything after that, and then collect the final urine at that same time the next morning.

This is where people often get mixed up. Many assume they are supposed to save the first urine on day one, but that is not how it works.

The first one gets tossed because it belongs to the time period before the collection officially started. The final one is saved because it falls within the 24 hours you are measuring.

Once you understand that, the whole thing makes a lot more sense. The jug may still annoy you, but at least it will not confuse you.


Follow Lab Instructions

Do not ask other patients online how to perform your urine collection, and do not assume their answer applies to you.

I see people do this all the time in Facebook groups (including mine) and it causes a lot of confusion. Different labs may have different instructions, different containers, different preservatives in the jug, different timing rules, and different ways they want the sample stored or returned.

People may mean well, but their instructions may be wrong for your lab. That can mess up your test and, in some cases, get your collection rejected altogether. Then you have done all that work just to hear, “Sorry, start over.”

No, thank you.

Please read the instructions that came with your own kit and follow those exactly. If anything is unclear, call the lab and ask them directly.


Eat and Drink Normally

Don’t start changing everything before the test. People often try to “do better” for the collection and they drink more water than normal, eat perfectly for a day or two, and hope the numbers look better.

But pretty numbers are not the goal. Honest numbers are.

We are not trying to impress the lab. We are trying to find out what is actually driving your stone risk. If your usual routine makes hydration tough, let the test show that. If your weekends look different from your weekdays, let the test show that too.

That is how we get useful information.


Don’t Miss a Sample

If you forget to collect even one bathroom trip, the results may be off. The lab is measuring what happened over the full collection period, and missing one piece can throw the whole picture off.

So if you are doing the test, commit to doing it carefully. Pick a day when you can realistically manage it. If you work a job where bathroom breaks are hard to come by, plan ahead or choose a better day to start.


Before You Start

Most kits include instructions on how to store the urine, and many recommend keeping the container cool. Read the directions before you start.

I know that reading urine collection instructions isn’t exactly how anybody wants to spend their afternoon. But this isn’t the time to freestyle it.

Follow the lab’s instructions exactly. If they give you a smaller collection device — called a “hat” by fellow nurses — use it and then pour the urine into the larger container. It makes the whole thing much easier.


The Results Create the Prevention Plan

Once the test is done, we can finally start making smarter choices.

Maybe your biggest problem is low urine volume, and hydration should be your first focus. Maybe your urine calcium is high, and sodium is playing a big role. Maybe oxalate is elevated, and we need to look at what you are eating every day. Maybe your calcium intake is too low, and that is making things worse.

The point is, now we know where to begin. We are no longer making blind guesses based on internet fear or random food lists.


Hire an Expert

If you already have results sitting in your portal and are thinking, what the heck does this mean, you are not alone. They are very complicated to interpret.

I have been helping people understand urine collection results since 1998. If you need help making sense of yours so you can better prepare for your doctor’s visit, work with me. I can help you understand what those numbers mean and what questions to ask next.


Ask for the Test

If your doctor has not ordered one, ask for it. Be direct.

Say: “I do not want to keep going through this. The urine collection will help show me why I am making stones, and I want that information so I can make the right diet changes.”

That is not being difficult. That is being smart.

If you want some guidance on how to ask for the test, read this.


Get Support

If you want support while going through the process, come join the conversation on my Facebook page. Just remember: community is great for encouragement, but your lab’s instructions are the boss.

I have YouTube and Spotify channels too—subscribe anywhere you listen to podcasts!


A urine collection is not glamorous, but neither is passing a stone.

One gives you answers. The other gives you pain, stress, and a bill you did not ask for.

So do the test correctly, keep your day normal, and let the results tell the truth.

That is how we stop guessing.

That is how we lower stone risk.

Your friend,
Nurse Jill

P.S. If your doctor orders a 48-hour urine collection instead of a 24-hour one, try to let those two days reflect real life, too. If possible, have one day look more like a workday and the other look more like a non-workday, because many of us eat and drink very differently depending on the day. That gives a more honest picture of your usual habits and your real stone risk.

When you’re ready, here’s how I can help you prevent kidney stones

Since 1998, I’ve helped thousands of patients prevent kidney stones. With my Kidney Stone Diet All-Access Pass, you get access to every prevention tool I’ve ever created:

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Jill Harris, LPN, CHC Avatar

About the Author

Jill Harris is a Licensed Practical Nurse (LPN) who specializes in kidney stone prevention. Her goal is to teach you what you need to know and, more importantly, how to put that knowledge to work so you can stop forming stones. For good.
  1. Loel Hofmann Avatar
    Loel Hofmann
    April 18, 2026

    Wouldn’t it be more accurate to collect samples separately during the 24 hour period and test each vs blending them all together or does it not make a difference? You are talking maybe 6-12 smaller samples vs ne large jug.

    Reply
    1. Jill Harris, LPN, CHC Avatar
      Jill Harris, LPN, CHC
      April 18, 2026

      Hi Loel,
      In order to get an accurate look of your day you need to collect it for the entire day. Separate samples during the day would show moment-to-moment ups and downs, but that is not what this test is validated for. Urine changes a lot based on meals, fluids, sweating, exercise, and time of day, so testing each pee separately would add a lot of noise and make routine interpretation harder. In stone prevention, the important reference ranges and treatment decisions are based on the full 24-hour collection, not a series of spot samples. Reviews on stone testing specifically note that untimed or spot urines are not recommended as a substitute for 24-hour excretion rates in stone formers.
      Hope that helps!
      Nurse Jill

      Reply
  2. JP Avatar
    JP
    April 18, 2026

    Hello Jill,

    I exercise a lot, and take supplements each day.

    If I request a 24 hour urine test from my urologist, will the supplements skew the test results?

    If so, is there any recommendations regarding the supplements?

    Thank you,
    JP

    Reply
    1. Jill Harris, LPN, CHC Avatar
      Jill Harris, LPN, CHC
      April 20, 2026

      Hi JP,
      yes some will. I write quite often about supplements. Here are some blog posts. https://kidneystonediet.com/vitamins-and-kidney-stones/
      https://kidneystonediet.com/which-vitamins-increase-kidney-stone-risk/
      https://kidneystonediet.com/chew-your-vitamins/
      Jill

      Reply
  3. Jeff Avatar
    Jeff
    April 18, 2026

    I’ve thought about the same thing as Loel. My past 2 or 3 Lab results have come back perfectly normal. Hopefully thanks to your advice. But if I am still forming them, my thought is that maybe one or two specific urinations get spiked then following ones from the day are diluting the whole thing down to normal. Either way, I always journal what I eat and drink during collections so I can go back and analyze things in the event of elevated results.

    Reply
    1. Jill Harris, LPN, CHC Avatar
      Jill Harris, LPN, CHC
      April 20, 2026

      Hi Jeff,
      If you labs are perfectly normal, you would NOT be making stones. Many are told their test results are normal, but are the lab’s reference ranges representing stone former risk or a more lenient value that could still lead to stones. If your doc doesn’t know where a stone formers values should be that could be the problem.
      Jill

      Reply
  4. Leanne Northrup Griffin Avatar
    Leanne Northrup Griffin
    April 19, 2026

    Excellent Jill. well done.

    Reply
    1. Jill Harris, LPN, CHC Avatar
      Jill Harris, LPN, CHC
      April 20, 2026

      Hi Leanne,
      I appreciate that coming from you. Thank you!
      Love, Jill

      Reply
  5. Bridget Ranalli Avatar
    Bridget Ranalli
    April 20, 2026

    Hi, Jill!
    First, let me off by saying how grateful I am to have found you over seven years ago. You have changed my life!

    My question is…How often do you recommend doing the urine sample?

    Reply
    1. Jill Harris, LPN, CHC Avatar
      Jill Harris, LPN, CHC
      April 20, 2026

      Hi Bridget,
      Here is the golden rule. Do the urine collection, go over results with doc, do whatever diet changes they ask of you, retest in 8 weeks. If everything is good, great. After that they should be done annually to make sure you still have a low stone risk. I cannot tell you how many people skip the annual and go on to making more stones.
      Jill

      Reply

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